The 29-year-old suffered the injury falling over a team-mate in the second half of the 1-0 Premier League defeat by Tottenham on Wednesday.
Barry visited a specialist on Thursday to discover the full extent of the injury and whether his rehabilitation could affect his World Cup chances.
England boss Fabio Capello names his provisional 30-man squad on Tuesday.
Capello will then cut his selection down to 23 players on 1 June following friendlies against Mexico (24 May) and Japan (30 May).
Mancini looks to improve 'all areas'
England begin their World Cup campaign in South Africa against the United States on 12 June.
Barry, who has won 36 caps, has become an established part of Capello's midfield and played in eight of England's qualifying games.
The midfielder has definitely been ruled out of City's final game of the season against West Ham on Sunday.
"I feel very sorry for him," said Manchester City manager Roberto Mancini.
"Gareth is a fantastic guy and a fantastic player.
"I don't know exactly how bad, maybe three to four weeks. I hope he can play in the World Cup but Fabio Capello and his medical staff must decide.
"He deserves to play in the World Cup"
Capello has previously suggested he would be reluctant to take any players who are not fully fit to South Africa.
606: DEBATE
Feel sorry for him. Wasn't picked by Sven, got back in the squad but missed out on Euro 2008 through no fault of his own, and now looks like he may miss the World Cup
EnglandGrim
The inclusion of Barry as a regular starter in England's midfield has been seen as key to solving the problem of getting the best out of Frank Lampard and Steven Gerrard
Barry has made 43 starts for City this season, scoring three goals as City qualified for the Europa League.
He joined City from Aston Villa in June 2009 for £12m, citing Villa's failure to qualify for the Champions League as one of his reasons for leaving.
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